Assembly of Long-read Sequencing

This hands-on practical workshop is an introduction to long read next-generation sequencing data and using SMRTLink.

Learning Objectives

  • Perform QC evaluation and filtering on long read next-generation sequence data
  • Use SMRTLink to assemble genome
  • Visualise assembly using SMRTLink
  • Assess genome quality using BUSCO and Augustus
  • Export genome data files for downstream analysis

Syllabus

  • Introduction to long read next-generation sequencing data
  • Differences in genome assembly from short and long-read next-generation sequencing data
  • Using SMRTLink to manage sample data, perform quality control filtering and for genome assembly
  • Visualisation of assembled genome in SMRTLink
  • Export of genome for downstream analysis and assessment of genome completeness

For more information contact training@qfab.org